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The Fy(a−b−) phenotype that occurs in Africans provides an explanation for the apparent absence of Plasmodium vivax in this region: this phenotype arises from homozygosity for the FY*B allele carrying a point mutation c.1-67T>C (rs2814778), which prevents Fy b antigen expression only in red blood cells.

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The Fy(a-b-) phenotype is the major phenotype in approximately 70% Blacks, but is very rarely found in other populations. This phenotype is characterized by the absence of the Fyb antigen on RBCs and its presence on non-erythroid cells. Duffy mRNA is not detected in the bone marrow of Fy(a-b-) individuals; however, it is detected in.

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Further, Fy(a-b-) phenotype in African origins is found in two-thirds of populations and rare in Europeans. Major antigenic frequencies are shown in Table 1. Additionally, a minor Duffy phenotype is the Fyx or [Fy(b+x)]. In this phenotype, the antigen is weakly expressed as Fyb and is determined by serological adsorption and elution techniques.

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The Fy (a−b−) phenotype, which is genotypically homozygous for the FY∗O allele, is common in African-Americans and West Africans (89). The coding sequence for FY∗B and FY∗O is identical (90). An SNP in the FY∗B 5′UTR leads to disruption of the GATA binding site for erythroid transcription factor (91).

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Duffy blood group system, classification of human blood based on the presence of glycoproteins known as Fy antigens on the surface of red blood cells, endothelial cells (cells lining the inner surface of blood vessels), and epithelial cells in the alveoli of the lungs and in the collecting tubules of the kidneys.The Duffy antigens Fy a (Fy1) and Fy b (Fy2) were discovered in 1950 and 1951.

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Further, Fy(a-b-) phenotype in African origins is found in two-thirds of populations and rare in Europeans. Major antigenic frequencies are shown in Table 1. Additionally, a minor Duffy phenotype is the Fyx or [Fy(b+x)]. In this phenotype, the antigen is weakly expressed as Fyb and is determined by serological adsorption and elution techniques.

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The 2C3-mAb recognizes the Fy6 antigen, a linear epitope on the first extracellular domain region of the Duffy glycoprotein adjacent to the antithetical Fy a /Fy b antigens, while the MIMA29-mAb binds to a region in extracellular domain 4 which is thought to carry the Fy 3 antigen. IgG isotype antibodies were used as a negative control, and.

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